Investigation of the relationship between hypertrophic cardiomyopathy and micro RNAS

Aim: Hypertrophic cardiomyopathy (HCM) is a heart muscle disease which primary causes myocardial hypertrophy. MicroRNAs (MiRNA) are small RNAs which involved in the regulation of protein synthesis in the cell and circulation. In this study, 11 micro-RNAs shown in the literature to be associated with cardiac hypertrophy were invastigated in peripheral blood by looking at the expression of miRNA. level whether it is specific to hypertrophic cardiomyopathy or not. Methods: 26 patients with HCM, 25 patients with hypertension and 24 patients of control group referred to our department between March 2012 and March 2013 were enrolled into this study. Blood samples were taken and 11 miRNAs‟ expression levels were analyzed by RT-PCR method. miRNA expression levels were compared between three groups. Results: miR-1, miR-21, miR-22, miR-23a miR-29, miR-30, miR-34, miR-133 and miR-199a expression levels were not difference between groups (p>0,05). miR-208 and miR-290 levels have not been evaluated because of inadequate expression. Conclusion: miR-1, miR-21, miR-22, miR-23a, miR-29, miR-30, miR-34, miR-133 and miR-199a expression levels did not show any difference in HCM, HT and control group. Key words: Hypertrophic cardiomyopathy, Micro RNA, real time PCR