Development of hyaluronic acid based and targeted nano carrier system

According to the World Health Organization (WHO) statistics, an estimated 8.8 million people die from cancer each year, and in 2020, these deaths are expected to exceed 13 million deaths. It has become important to work on targeted nano-carrier systems to eliminate the disadvantages of current cancer drugs. In this study; a hyaluronic acid (HA) based nano-carrier liposomal structure has been developed that will target CD44, the tumor cell surface receptor. The HA, which was found to have a molecular weight of about 95 kDa, was purified and stabilized by using Sephadex G-75 from Streptococcus equi culture and transformed into a size of 5kDa by the immobilized Hyaluronidase (Hyase) column system. HA characterization was performed by Gel Permeation Chromotography (GPC) and Hexadecyltrimethylammonium bromide (CTAB) method. The purified HA was converted to the MATyr-Ru(bipy)2-HA complex to be used for targeting by the ANADOLUCA method and ''Green Fluorescent Protein'' (GFP) was used both as a secondary agent and for imaging. siRNA encapsulated liposome was synthesized by thin film hydration and sonication method using 1,2-Dioleoyl-sn-glycero-3-phosphocholine (DOPC), 2-Methacryloyloxyethyl phosphorylcholine (MPC) and Cholesterol (Chol). Scanning Electron Microscopy (SEM) and size and zeta potential analyzes were performed for characterization. The liposome was found to be about 90 nm in size. In conclusion, the obtained nano-carrier liposomal structure is thought to be a promising approach for both imaging and treatment of cancer cells.