Hücre yüzeyi proteinlerinin sitokinezdeki rollerinin belirlenmesi

Cell division requires many sequential and coordinated actions of chromosomes, cell cycle machinery, cytoskeleton, and cellular membranes. These events have been extensively studied in many cell biology laboratories. Among those, how cell surface components and intracellular cell cycle machinery communicate, especially during cytokinesis, is much understudied. In this project, we aim to determine the roles of cell surface proteins during cytokinesis. Previous studies in the Eggert lab have shown that Glypicans 3 and 5 might have a role in cytokinesis since silencing their expression causes multinuclear cells. Glypicans are members of heparan sulphate proteoglycans that are highly conserved among eukaryotes. They are covalently attached to the plasma membrane via GPI anchors where they entirely face the extracellular matrix. In this thesis, I determined cellular localization of Glypican 3 and 5 during cell division and characterized their cell division phenotype. We performed a mass spectrometry-based proximity interactome analyses of Glypicans 3 and 5 at interphase and dividing cells. Our study revealed that Glypican 5 might have important roles in cortical actin stability and midbody abscission. In addition, we identified several proteins as Glypican 3 and 5 interactors that might give insights about the molecular mechanisms underlying their role in cytokinesis.