Supercritical CO2 extraction of date palm (Phoenixdactylifera L.) pollen and investigation of fatty acidcompound by GC-MS

In this study, Supercritical CO extraction with various solvents, pressure and heat values on date palm (Phoenix dactylifera L.) pollens, SEM studies demonstrating the effects on the pollen structure and GC-MS analysis of the extracts were conducted. Dominant fatty acid of Supercritical experiments was Hexadecanoic acid (CAS) (%42.27), followed by 9,12 Octadecadienoic acid (%10.95), 9-Hexadecanoic acid (%7.62) and Linoleic acid ethyl ester (%4.99) respectively.Extractions and GC-MS anlaysis results showed 28 compounds that hasn't been found on previous studies of date palm pollen. That includes a new compound, 1,5 dimethyl-6-(1,5-dimethylhexyl)-15 (%41.40) whose moleculer properties and bioactivity are unknown to our knowledge. Pregna-5,16-dien-20-one was found with %24.64 amount, which has anti-cancer activities. After Supercritical CO2 extraction experiments, changes on pollen morphologies were determined with SEM microphotographies of pollens. Bioactivites and groups of the compounds obtained as a result of extractions are given.