Identification and analysis of PEA3 transcription factor target promoters

Transcription factors are the proteins which regulate the expression of the target genes. Improper alterations in the activity of transcription factors may lead to severe diseases.The PEA3 group of transcription factors belong to the ETS family and bind to conserved core DNA sequences 5'-GGAA/T -3' It has been showed that, PEA3/ETV4 transcription factor participates in the motor neuron connectivity and dendritic patterning. In addition to this, it is believed that, PEA3/ETV4 protein is able to regulate the genes involved not only in neuronal differentiation, neuronal development and axonal pathfinding but also in metastatic cases of several cancer types, especially lung and prostate cancer.In this study, we aim to identify the downstream targets of PEA3/ETV4 transcription factor to reveal its signaling pathway. For this purpose, we merged the in silico search and molecular biology techniques. Meanwhile, we developed a novel bioinformatics tool which screens and analyze candidate target promoters for PEA3/ETV4 and other transcription factors.