The effect of Epigallocatechin-3-Gallate on nrf2, Nf-kB and TNF-alpha protein signaling pathways against muscle tissue damage

Cisplatine, which is used as an effective drug in cancer patients, has become a popular topic in studies conducted in recent years. It is known that cisplatin terminates the vital activities of healthy cells as well as cancer cells. Epigallocatechin-3-Gallate (EGCG), a member of the catechin group, is a natural antioxidant known to have health-promoting benefits. Catechins, due to their regulatory effect on apoptosis and autophagy, offer an increasing interest in having positive health effects on various diseases. The aim of this study is to investigate the effect of EGCG on Nrf-2, Nf-κB and TNF-α protein signaling pathways against muscle tissue damage caused by cisplatin. In the study, 4 groups were created and a total of 28 male rats were used. The experimental period was 28 days (4 weeks). The protective and healing effect of EGCG against the damage caused by cisplatin in muscle tissue has been investigated. catalase (CAT) activity and malonaldehyde (MDA), and glutathione (GSH) levels were measured in the spectrophotometer. Expression levels of Nrf-2, Nf-κB and TNF-α proteins were determined using the Western blot method. Compared to the cisplatin-administered group, it was determined that while the MDA level decreased in the EGCG+Cisplatin-administered group, there was an increase in CAT activity and GSH levels. It has been found that EGCG used for therapeutic purposes reduces the expression of Nrf-2, Nf-κB and TNF-α proteins. In the histopathological examination, it was observed that muscle tissue damage occurred in the cisplatin group, while the damage decreased in the EGCG + Cisplatin group. Our findings show that EGCG can prevent toxicity due to its anti-inflammatory effect against tissue damage caused by cisplatin.