Contribution of katp channel to the possible antinociceptive effect of o-coumaric acid

Pain is an uncomfortable feeling that affects daily life and is experienced by every person. Phenolic acids, which have antioxidant properties, are natural bioactive compounds that are noteworthy for pain-relieving activity. There is no study yet in the literature showing that o-coumaric acid, which is an isomer of coumaric acid and is commonly found in plants, has an analgesic effect. In this thesis study, it was aimed to investigate the analgesic effectiveness of o-coumaric acid at doses of 25, 50 and 100 mg/kg, i.p. by applying the acetic acid-induced writhing test in mice. The antinociceptive effect of o-coumaric acid had compared with 30 mg/kg diclofenac which used as a positive control drug. Potassium channels are the most popular, widely distributed, and diverse class of ion channels in bodies, opening of these channels results in analgesia. To investigate the role of KATP channel pathway in o-coummaric acid mechanism and to determine the contribution of GS on activation potassium channel 10mg/kg, i.p. of glibenclamide as KATP channel blocker and 20mg/kg, i.p. of methylene blue as GS blocker were used. According to the obtained data, it was determined that the KATP channel plays a significant role in analgesic effect of o-coumaric acid, while sGMP does not, and that the activation of KATP channels occurs through different mechanisms. In summary, it is believed that o-coumaric acid provides notable peripheral analgesia and further research based on this study will provide an efficient approach.