Development and validation of HPLC ultraviolet detection method for quetiapine fumarate estimation in pharmaceutical formulation

For determining quetiapine fumarate, a validated, quick, specific, easy, inexpensive, and accurate high performance liquid chromatography ultraviolet detection HPLC UV approach that may be utilized for regular quality control evaluation and in vitro research has been established. Due to its peak symmetry, resolution, and quick analysis time, the selected column Raptor C18 250 x 4.6 mm provided the best separation. Flow rate was 1 min-1 and the injection volume was adjusted to 10 L, the ambient temperature was 25 °C. The mobile phase was containing a buffer of 60% disodium phosphate Na2HPO4 and acetonitrile 40% ACN at pH 6. The analyte was detected using a UV detector at 225 nm. The developed method fulfilled all of the SST's approval requirements. The method has been thoroughly examined, and the results indicate that it may be used for the estimation of quetiapine fumarate in pharmaceutical formulations and in vitro studies.