The investigation of some signal transduction biomolecules in Triticum aestivum L. (wheat plant) exposed to drougt stress

In this study, the effects of drought stress and externally applied different concentrations of sodium nitroprusside (SNP) and hydrogen peroxide (H2O2), on nitric (NO) levels, nitric oxide synthase (NOS) activity, cyclic guanosine monophoshate (cGMP) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), antioxidant enzyme activities, malondialdehyde (MDA) content, total soluble protein concentrations, hydrogen peroxide (H2O2) and relative water content of the tolerant (Triticum aestivum L. Doğu-88) and sensitive (Triticum aestivum L. Yıldırım) wheat plant?s root and leaf tissues were investigated. Both resistant and sensitive wheat crops grown in drought stress conditions, be involved in signal transduction and antioxidant enzyme activities and functions of biomolecules and possible changes caused by SNP and H2O2 signaling system were tried to explain the mechanism of the experimental system. In this study, polyethylene glycol (PEG) by application of drought stress created in the wheat plants. In this study, PEG by application of drought stress created in the wheat plants. Wheat plants grown in Hoagland culture solution 15 days, 2, 4 and 6 days SNP and H2O2 (10 µM, 100 µM, 1000 µM) applications drought (PEG) application and implementation of drought at the same time SNP (PEG+SNP) and H2O2 (PEG+ H2O2) were used. The control group was grown in Hoagland culture solution.As a result, 10% of the Doğu-88 and Yıldırım varieties to drought, various concentrations of SNP and SNP+PEG and PEG+ H2O2 H2O2-treated root and leaf tissues, nitric oxide (NO) levels, nitric oxide synthase (NOS) activity, cyclic guanosine monophosphate (cGMP) levels, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), antioxidant enzyme activities, malondialdehyde (MDA) content, total soluble protein amounts of hydrogen peroxide (H2O2) and relative water content and treatment days groups differ depending on the take. Relative water content and the amount of soluble protein in particular have increased Yıldırım variety. The result from our study, generated against the plant?s roof and leaf tissues, signal transduction, and responses to stress conditions found to be different. In our study, the antioxidant enzyme activities increased in Yıldırım variety and decreased in Doğu-88 variety. The assumed NO plays a role in signal transduction, cGMP and H2O2 were increased in both varieties but more increased levels detected in Doğu-88 variety. Signal transduction and antioxidant enzyme activities and NO donor SNP on the H2O2 and drought-sensitive wheat varieties containing the observed effects of stress increases the protection of sensitive varieties was determined to play a role.