Prebiotic and antibiopilim activities of exopolisacharides from Lactobacillus rhamnosus ACS5

In this study, it was aimed to reveal the prebiotic potential of lyophilized exopolysaccharide (L-EPS), which was obtained from Lactobacillus rhamnosus ACS5 by determining the growth regulatory effect, gastric acid resistance and prebiotic score activity by fermentation by potential probiotic microorganisms. In addition, antibiofilm and antimicrobial activities of probiotics, prebiotics (L-EPS), postbiotics, and parabiotics were determined. It was observed that L-EPS positively affected the growth of Lactobacillus rhamnosus EDS4, Pediococcus acidilactici 5008, and Saccharomyces cerevisiae BD21 strains. The highest growth regulatory effect of L-EPS was designated in S. cerevisiae BD21 (log 10.26 cfu/mL). P. acidilactici 5008 and Enterococcus feacalis ATCC 29212 pathogen strains showed the highest prebiotic score activity of 2.30 and 2.92 at 24 and 48 hours, respectively. It was determined that the hydrolysis of L-EPS in gastric acid is lower than that of inulin. It was found that the biofilm inhibition rates of the tested concentrations of L-EPS against Escherichia coli ATCC 11229 were higher than Listeria monocytogenes ATCC 7644. Antibiofilm activity of the ACS5-postbiotic was observed to be 20.3±11.17% against E. coli ATCC 11229, while it was found to be 10.95±4.03% against L. monocytogenes ATCC 7644. It was determined that parabiotics showed 27.75% and 65.25% inhibition on biofilms produced by E. coli ATCC 11229 and L. monocytogenes ATCC 7644 intestinal pathogens, respectively. While it was designated that the antimicrobial effect of probiotic prebiotics, symbiotics, and postbiotics on E. coli ATCC 11229 test bacteria was higher than L. monocytogenes ATCC 7644, but no antimicrobial effect was detected in parabiotics. As a result of the studies, it was foreseen that L-EPS obtained from L. rhamnosus ACS5 has the potential to be used as a prebiotic.