Cytotoxic effect of lamium purpureum L.essential oil on different cancer cell lines

In this study, the cytotoxic effect of Lamium purpureum L. Essential Oil on different cancer cell lines was investigated. In our study, human endothelial cell line HUVEC (Human Umberical Vein Endotehlial cells), SW-480 (Human colon cancer), PC3 (Prostate Cancer) and Hep3B (Human liver cancer) cell lines were studied. The effects of L. purpureum essential oil on cell viability of Hep3B, PC3, Sw480, Huvec cell lines exposed for 24, 48, 72 h were determined by MTT. The effects of L. purpureum essential oil on cell migration and colony formation on liver cancer cells were determined by clonological analysis. For the wound healing test, photographs were analyzed with the Image J program. Analysis of the chemical composition was carried out by purchasing service at Kastamonu University Central Research Laboratory Application and Research Center using the GC-MS (Gas Chromatography-Mass Spectroscopy) method. Among the identified compounds, 2-pentadecanone, 6,10,14-trimethyl 19.35%, phytol 13.10% and germacrene-D 5.79% were found to be the main components. When the antiproliferative effect of L.purpureum oil was examined on the Hep3B cell line, a high cytotoxic effect was observed at all concentrations after 24 hours and 72 hours of application, compared to the control group. The effect of L.purpureum oil on tumorigenic properties on Hep3b cells was determined by scratch assay and colony formation test. In the colony formation analysis of Hep3b cells, although a difference was observed in the number of colonies between the control group and the group treated with L.purpureum oil, this difference was not found to be statistically significant. When the results of the wound healing test, in which cell migration was analyzed in the Hep3b cell line, were evaluated statistically, it was observed that the mobility or migration of the cells decreased and this change was statistically significant. It is anticipated that the data obtained as a result of this study will shed light on future studies.