Investigation of free radical metabolism in septic rat's renal tissues treated with lipopolysachharide; effect of Vitamin D

Sepsis, a multiple organ dysfunction syndrome, is a common cause of morbidity and mortality in the intensive care unit. LPS-induced excessive immune response is associated with hypovolemia, shock and multi-organ damage in sepsis. Excessive immune response causes multi-organ damage by increasing oxidative stress. In last years, studies on the immune regulatory and antioxidant effects of vitamin D demonstrated its protective role. In this study, we aimed to investigate the effects of VitaminD on free radical metabolism in LPS injected rats. 24 female wistar albino rats (weigt 250-350 g.) were divided into 4 groups randomely. 1) Control , 2) Sepsis, 3) Sepsis+vitamin D, 4) Vitamin D. Sepsis was induced with single intraperitoneal injection of LPS E.coli (O111-b4) 16 mg/kg. Vitamin D was given 2 mg/kg 25(OH) single dose via gavage (in sunflower oil) for 3 days. Rectal body temperature was measured in rats. Renal function tests (BUN, creatinine) were analysed in serum by Integra 800. Tissue catalase (CAT), superoxide dismutase (SOD), glutathione peroksidase (GSH-Px) and glutathione-S-transferase (GST) levels were analysed kinetically. Additionally rat renal tissues were analysed histopathologically. SOD and GSH-Px activities were not significantly different between the groups. CAT activities were depressed in all groups compared with control group, this inhibition was mainly watched in the sepsis+vitamin D group. GST activities were depressed in sepsis ve sepsis+vitamin D group. BUN and creatinine levels were elevated in sepsis and sepsis+vitamin D group. While control and vitamin D groups showed normal histological structure, inflammation, expansion in bowman capsule and degeneration in sepsis and sespsis+vitamin D groups. In conclusion, we found that vitamin D treatment in sepsis has no protective role against renal toxicity effects on liver. However treatment vitamin D in sepsis have inhibitory effect on antioxidant enzymes which use H2O2 and GSH metabolism. We think that more detailed assessment of this subject is necessary in terms of glutathione and enzymes using glutathione. Key words: renal toxicity , sepsis , vitamin D , antioxidant enzymes