Mantle hücreli lenfomalarda özgün tanı, prognoz belirteci veya tedavi hedefi olma potansiyeline sahip uzun kodlamayan RNA'ların tüm transkriptom dizi analizi yoluyla belirlenmesi

Mantle cell lymphoma (MCL) is a B-cell non-Hodgkin's lymphoma (NHL) which is considered as an incurable lymphoid neoplasm with a continuous relapse pattern and a median survival of only 3-5 years. Therefore, there is an urgent need to elucidate the MCL disease pathobiology and identify novel biomarkers and therapeutic target in order to optimize the treatment and improve the prognosis of MCL patients. Long noncoding RNAs (lncRNAs) have been shown to involve the development of many cancers and some of them are used as novel biomarkers. However, there are very few reports of lncRNAs that are related to the MCL pathogenesis and prognosis. In this study, we aimed to identify lncRNAs that have the potential to be novel diagnostic, prognostic biomarkers or therapeutic targets in MCL through whole-transcriptome sequencing. We examined the lncRNA and also mRNA expression profile of 18 MCL samples and compared with 3 control samples. A total of 1149 significantly differentially expressed lncRNAs were detected with 322 of lncRNAs upregulated and 827 of lncRNAs downregulated in MCL. We analyzed some selected lncRNAs in MCL patient tumor samples - for cross-validation of WTS results - by qRT-PCR. We also performed pathway analysis to interpret the differential expression data. And we found that upregulated mRNAs were mainly involved in angiogenesis and pathways in cancer. We examined the diagnostic value of differentially expressed AC010983.1, LINC01268, PWRN2, MAGI1-IT1 lncRNAs and SOX11, EZH2 and IL33 mRNAs by ROC curve analysis. Despite a high area under the curve value of selected transcripts, according to the log-rank test of Kaplan Meier curves, p values were not significant ( P > 0.05). However, our present study showed the potential value of significantly differentially expressed lncRNAs and mRNAs on MCL patient prognosis which should be further investigated with increased sample number and functional assays.