Zinc biosynthesis from blue butterfly (Clitoria ternatea) extract oxide nonaparticles are antimicrobial, antidiabetic and antioxidant in vitro evaluation of activities

The synthesis, characterization, research and application of bio-functional biomedical nano-sized materials for biomedical applications is of vital importance. With the green synthesis method, nanostructures of different shapes and sizes are synthesized using biological materials called environmentally friendly, non-toxic and safe reagents. The aim of this study was to evaluate the antimicrobial, antioxidant and antidiabetic activities of Clitoria ternatea extract-mediated synthesized zinc oxide nanoparticles (MK-ZnO NP) for use in biomedical applications. Zinc oxide nanoparticles synthesized by green synthesis using Clitoria ternatea aqueous plant extract exhibited an absorption peak at UV-vis 362 nm and a characteristic absorption peak at 1 keV due to surface plasmon resonance in EDX analysis. Zn (48.9%) was determined in the elemental composition of MK-ZnO NPs. The average crystal size of ZnO NP was calculated as 16.32 nm. MK-ZnO NPs showed significant antibacterial activity against Staphylococcus aureus, Enterococcus faecalis and Escherichia coli species. DPPH radical scavenging activities of MK-ZnO NPs (50-2000 μg/mL) were found to be the lowest 9.4% and the highest 78.1%. The IC50 dose was calculated as 623.48 μg/mL. The percentage of inhibition of α-amylase enzyme activity by MK-ZnO NPs (1250- 10000 μg/mL) was found to be the lowest 70.6% and the highest 94.3%. The IC50 dose was calculated as 502.61 μg/mL. In conclusion, zinc oxide nanoparticles successfully synthesized using Clitoria ternatea are promising as an agent that can be used in biomedical fields in terms of antimicrobial, antioxidant and antidiabetic activity.