Mazı arısı Andricus quercustozae (Hymenoptera:Cynipidae)'nin tür içi mtDNA varyasyonu ve filogenetik yapısı

Intraspecific mitochondrial DNA variation and phylogenetic relationships of Andricus quercustozae haplotypes from Turkey was studied using PCR-RFLP method. An 2540 base pair mitochondrial DNA region covering ND4, ND4L, tRNAThr, tRNAPro, ND6, cyt b gene and a second 1800 base pair fragment comprising the ATPases (6, 8) and COIII amplified by PCR were digested with restriction enzymes including HindIII and HinfI, HaeIII, HindIII, EcoRI, respectively. A total of 28 haplotypes were detected among 95 individuals collected from 16 populations. The estimated average haplotype and nucleotide diversity within populations were 0.45 and 0.05, respectively. The highest nucleotide divergence estimate among the analyzed oak gall wasp populations was 9.0% indicating an ancient split between A. quercustozae haplotypes.Dendrogram obtained using PHYLIP program depicting the phylogenetic relationships of haplotypes indicated that there was a significant relationship between geographical distribution of haplotypes and their clustering. AMOVA analysis for estimation of the partitioning of genetic differentiation at different hierarchical levels was statistically significant. Analysis of variance revealed the highest genetic variance (38%) was present within groups, a significant partitioning of variance (25%) was found among groups and an intermediate level of variance was present within populations (35%).Overall, the present study indicates that the oak gall wasp haplotypes found in different populations of A. quercustozae have significant amount of genetic diversity and they form geographically significant groupings. It can also be concluded that varied topography of Turkey plays an important role in shaping the current population genetic structure of the oak gall wasp species.