Media optimization, scale-up and pilot scale production of Bacillus sphaericus MBI5 used for mosquito control

Pest and disease vectors cause losses in the production of major crops and transmit mosquito-borne diseases worldwide. To reduce disease pathogen transmitting pathogen vector control should be properly designed with appropriate the control methods and long-term strategies. Chemical insecticides are applied in agricultural lands and on animals therefore they can cause easily spread different fauna via some physical effects such as wind, rain, motion of insects. Even if it is out of aim, these applications draw individually and collectively for the acute, subacute, and chronic poisoning with mutagenic, carcinogenic and teratogenic effects on humans, animals, bees, fish and some beneficial insects. In the world today, biopesticides are safe and environmental-friendly solution in order to control insect pest to prevent plant damage and infection diseases. Bacillus sphaericus (Bs) toxin has high specificity against insects of the dipteran family which are responsible vector-borne diseases. Bs produces large amount of toxin protein during sporulation phase and this protein gathers in the sporangial form as parasporal body. In the current study, a novel strain (Bs MBI5) has been produced based on power input scale up strategies. In this thesis, we have focused on fermentation development of novel Bs MBI5 strain producing highly effective biopesticide. The research achieved significant success in media and process optimization, process scale up and large scale production. This study is the first Bs cultivation research that applies using power input way to scale up of fermentation, and high yield fermentation using waste product.