Mercaptopropiyonik asit kullanan dopamin tespiti ve elektrod yüzey modifikasyonu için sisteamin

This research work emphasizes on main objective of dopamine detection using electrochemical detection analysis. Dopamine is an electrochemical neurotransmittor and is an essential part in human body for many decisions. It can also be described as a chemical released by neurons (nerve cells) to send signals to other nerve cells. The interest in dopamine was stimulated due to several physiological and neurological diseases such as Parkinson's disease, schizophrenia, obsessive compulsive disorder and many drug addictions. Gold electrodes are often not suitable for the dopamine measurements as dopamine creates a non conducting polymer layer on the surface of the electrodes, which leads to the increased amount of electrode passivity with the gradual increase in voltammograms measurement. This work is presented to contribute in a better understanding on the comparative study of targeted dopamine detection with two surface modifications for Au electrodes with mercaptopropionic acid (MPA) and cysteamine. The results are further characterized with the impedance spectroscopy (EIS) and cyclic-voltammetry (CV) for providing a comparison of dopamine detection for thermally bonded and ultrasonically welded microfluidic chips respectively. Moreover, the effects of selecting optimized tubing, bonding techniques, and cleaning methods of the devices with KOH solution played crucial role for improvements in dopamine detection which are observed in results. Also a comparison for the modification with unmodified chips, and finding the unknown concentration of dopamine solution using flow injection techniques has also been illustrated.