Optimization of desferrioxamine biosynthesis through co-culturing of micromonospora craterilacus NA12 and streptomyces SP. MK37H strains

Iron accumulation in the human body has strong oxidant effects that can cause tissue damage. The excessive iron accumulation creates a favorable environment for various diseases in the heart, liver, lungs, brain, bone marrow, and endocrine organs. The iron accumulated in the body is removed by desferrioxamine from the body by chelating or binding to it. Desferal®, used especially in treating dialysis patients in our country, is a drug containing desferrioxamine active substance commercially released by Novartis Pharma AG (Basel, Switzerland) and is now imported from abroad. This study was designed to optimize siderophore production from strains with desferrioxamine biosynthetic gene clusters by bioinformatic analysis from native microbial sources (Micromonospora craterilacus NA12, Streptomyces sp. MK37H). In the study, four different media, GPHF, GYM, N-Z-Amine, and TYG agar, were used to activate microbial sources in the appropriate environment. Microorganisms developed in N-Z-Amine medium in which they show optimum growth were inoculated into LB and TYG broth prepared using different carbon sources (glucose, maltose, sucrose, and starch) for desferrioxamine siderophore biosynthesis and grown in a shaking incubator at 28°C for 7 days. After centrifugation of the culture broth, the presence of siderophores was shown spectrophotometrically (630 nm) following the liquid CAS assay. Desferrioxamine was used as a standard, and the % siderophore efficiency was calculated. Based on the medium with the highest production of siderophores, it was aimed to increase the production of siderophores by culturing the strains with Nocardia sp. GTS18. As a result of the optimizations made during the research, Micromonospora craterilacus NA12 and Streptomyces sp. MK37H showed better growth in TYG broth compared to the LB medium. It was concluded that a higher yield of desferrioxamine siderophore was obtained by co-culturing and using starch as a carbon source in a TYG medium.