Mikroakışkan biyoreaktörlerde ß-tcp esaslı doku iskeleleri kullanılarak osteojenik niş geliştirilmesi

Traditional bone tissue engineering (BTE) applications often aim to develop therapeutic solutions. Most of these efforts are circled around a biomaterial to provide optimum results. However, reliable generation of biological properties of BTE biomaterials suffers from the poor recapitulation of in vivo systems in laboratory conditions. Much of problems stem from poor mimicry systems. Therefore, a physiologically relevant in vitro system, which can provide a total mimicry of native environment and thus assist accessing the dynamic behavior of osteogenic environment is needed. In this study, a functional osteogenic niche model, which can perform bone resorption/secretion was intended. To achieve this, primary mouse bone marrow cells, a tailored biomaterial and microfluidic bioreactors was used. Using dynamic co-culturing methods, this thesis discusses a simpler and robust approach to functionalize the osteogenic niche while providing an in vivo-like microenvironment. The developed platform achieved a scaffold that is tailorable for any bioreactor setup, shapes and volumes with desired porosity percentages. The ß-TCP was subjected to material characterization methods to confirm its resemblance to native inorganic bone. For osteogenic niche, freshly isolated mouse bone marrow mesenchymal stem cells and monocytes/macrophages was used to form basic cellular components of the bone. The osteogenic niche was seeded on the scaffold and placed in a bioreactor. Scanning electron microscopy analysis showed that after 21 days of culture cell extracellular matrix was secreted throughout the scaffold volume. Moreover, a significant increase in osteocalcin, ALP and cathepsin-K proteins was observed as an indication of formation of osteoblast and osteoclast cells.