Mikroakışkan çip-üstü-organ modellerinde hücre tutunması için pdms yüzey fonksiyonelliği

The inherent hydrophobicity of Polydimethylsiloxane (PDMS) poses significant challenges for its use in cell culture applications, particularly in organ-on-chip technology. This study presents a novel, versatile, and biocompatible surface treatment method for polydimethylsiloxane (PDMS), designed to significantly improve its cytocompatibility and shear stability in organ-on-chip applications. Four tailored surface functionalization protocols were systematically explored using 3- (Trimethoxysilyl)propyl methacrylate (TMSPMA), optimized to enhance wettability, surface roughness, and chemical functionality, leading to robust and sustained cell adhesion. The efficacy of these treatments was confirmed via comprehensive surface characterizations of contact angle measurements, scanning electron microscopy (SEM), atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). The changed hydrophilicity supported robust cell adhesion, proliferation and viability that were determined using three distinct human cell types: HUVECs, MCF-7, and Humanderived induced pluripotent stem cells (iPSCs). Importantly, our findings show that the treated PDMS supports long-term adhesion and proliferation under both static and dynamic microfluidic conditions, with minimal cell detachment even under flow rates corresponding to physiological shear stress (~1.5 dyne/cm²). Numerical simulations further validate the experimental flow conditions, strengthening the model's physiological relevance. This study underscores the effectiveness of TMSPMA treatment in improving PDMS compatibility for cell culture, offering an accessible, tunable, and scalable solution for researchers aiming to create more reliable and biologically relevant microfluidic systems.