Biodetermination applications with microfluidic based systems

The aim of this study is to develop early diagnosis systems for different diseases with direct ink printing technique, microfluidic channel system or lens using a 3D bioprinter. In this context, new generation flexible microfluidic channels were created using PDMS encapsulation and Pluronic F-127 bio-ink. Thus, personalized determination systems have been developed by requiring very little samples in micro and nano sizes. The determination of alpha-crystalline with this method, besides being non-invasive, has been rapidly determined spectroscopically. In the second stage of this thesis, after the modifications made on the PDMS and SE-1700 based lenses created by using a 3D bioprinter, glucose determination from the tears was performed with a colorimetric method. With the glucose molecules in the tears, GOx is oxidized and Hydrogen Peroxide is released. Thus, TMB, the substrate of HRP, is oxidized and a blue color formation is observed depending on the concentration. Thus, by intensifying the blue color formed, a colorimetric method of glucose determination from tears can be performed. In the last stage of this thesis, after the MATyr-Ru(bipyr)2-Biotin and TMSPMA modification, which were synthesized according to the ANADOLUCA method into the microfluidic channel, phospho-tyrosine determination for early diagnosis of cancer was carried out depending on the concentration. After the diagnosis process was carried out in the microfluidic channel with the enzyme-conjugated antibody structure modified on the surface, it was carried out at 450 nm with a microplate reader.