Model bakteri hücre sınırlarında protein-DNA ayrılma kinetikleri ve kromozom organizasyonu

Transcriptional initiation and repression require the temporal interactions of transcription factors with DNA. Recent experiments showed that the interaction lifetime is crucial for transcriptional regulation. Relevantly, in vitro single-molecule studies showed that nucleoid-associated proteins (NAPs) dissociate rapidly from DNA through facilitated dissociation (FD) with the increasing phase-solution protein concentration. Nevertheless, it is not clear whether such a concentration-dependent mechanism is functional in bacterial confinement, in which NAP levels and the 3D chromosomal architecture are coupled. Here, we employ extensive coarse-grained molecular simulations, where we model the dissociation of specific and nonspecific dimeric NAPs from a high-molecular-weight circular DNA polymer in a rod-shaped structure constituting the cellular boundaries. Our simulations indicate that the peak cellular protein concentrations result in highly compact chromosomal conformations. Such compactions lead to shorter DNA-residence times but only for NAPs demonstrating sequence-specificity, such as the factor for inversion stimulation (Fis). On the other hand, the dissociation rates of nonspecific NAPs decrease with the increasing protein concentrations, exhibiting an inverse FD behavior. Another set of simulations utilizing restrained chromosome models reveal DNA-segmental fluctuations as the cause of this reversed response, suggesting that moderate chromosomal compaction promotes protein dissociation. Together, our findings suggest that cellular quantities of structural DNA-binding proteins could be highly influential on their residence times and the chromosome architecture.