Determination of Nebivolol in its tablets with capillary electrophoresis

Nebivolol is a third-generation ß1-selective ß-blocker that is approved for the treatment of hypertension. In this study simple, accurate and rapid capillary electrophoretic method was developed and validated for the quantitation of nebivolol hydrochloride in tablets. 3,4-Dihydroxybenzyl amine was used as an internal standart. The effect of the buffer concentration, buffer pH, amount of organic solvent and buffer type was systematically investigated on the separation. The optimum conditions determined were as following, 40 mM sodium acetate and 66 mM glacial acetic acid (pH 4.2) consisted of %10 (v/v) methanol for run buffer, 25 kV for separation voltage and 0.5 sec for injection time. Analysis was carried out with UV detection at 200 nm. The migration times were 4.2 min for internal standard and 5.3 min for nebivolol hydrochloride. The method was validated in terms of accuracy, precision, linearity, selectivity and robustness. The method was linear over the range of 9.72 ? 49.51 µg/mL. Limit of detection and quantification were 2.61 and 7.91 µg/mL, respectively. The method was applied to tablet dosage forms and nebivolol hydrochloride content of the tablets was found 5.166 ± 0.087.Key Words: nebivolol, capillary electrophoresis, 3-4 dihydroxybenzyl amine, tablet