Determination of nebivolol in pharmaceutical formulation using high performance liquid chromatography with fluorescence detection

A rapid, simple, specific and sensitive high performance liquid chromatographic method with fluorescence detection was developed and validated for the determination of nebivolol in tablets in this study. Optimum conditions were found to be as flow rate of 0.9 mL/min, injection volume of 10 µL, detection wavelength of 282 nm for excitation and 310 nm for emmision in a Gemini C6 phenyl column using mobile phase of acetonitirile:formic acid (50 mM), (25:75, v/v). The method was linear over the concentration range of 1.03x10-6-5.15x10-6 M (0.42-2.09 µg/mL). It was shown that the mehod was produced accurate and precise results in this concentration range. Limit of detection and limit of quantitation were calculated as 18.5 ng/mL and 55.9 ng/mL. It was determined that the nebivolol content of commercial tablets was in the limits of The United States Pharmacopeia, 2006 by using proposed high performance liquid chromatographic method.