Determination of resistance genes against anthracnose disease (Ascochyta rabiei) and developing of specific markers

Environmental problems occurring in the world, lack of production, cost of animal products etc. Due to situations, the need for vegetable origin has increased. In order to meet this need, it is preferred to use dry grains of grain legumes due to the high amount of protein, vitamins, minerals and amino acids. Chickpeas are the fourth most preferred grain legumes in the world after beans, soybeans and peas. Chickpea is susceptible to ascochyta blight caused by Ascochyta rabiei (Pass) Lab. This is the most devastating chickpea disease. The disease affects all the air parts of the plant, causing necrotic lesions in stems, skins, broad beans and seeds. Fracture of stems and leaf stems is also an important symptom. In the area, infected plants are often seen as spots that reflect foci where the pathogen spreads. These spots are irregularly sized, ranging from light brown to blackish dark brown and are surrounded by the yellow circle. It can cause losses of up to 100% when favorable conditions occur for the pathogen. In order to spread the disease, it is sufficient to have a presence in the region raised. In our country, quite a lot of losses are given against Ascochyta rabiei pathogen. In our study, the localization of the sequences that may be resistant to anthracnose blight resistances genes (NBS-LRR gene family), and specific markers were designed using nucleotide sequences. Through the designed markers, the presence or absence of sequences that may be resistance genes in the genomes of the thirty-eight varieties / hybrid chickpeas obtained from the Eskişehir Crossing Agricultural Research Institute. As a result of the data we obtained, it is aimed to increase the studies on the use of beaten individuals in the fight against Chickpeas Anthracnose blight.