Investigations on spectrum and frequency of induced mutation in chickpea (cicer arietinum L.)

ABSTRACT INVESTIGATIONS ON SPECTRUM AND FREQUENCY OF INDUCED MUTATION IN CHICKPEA (Cicer arietinum L.) Cengiz TOKER Ph. D. Thesis in Department of Field Crops Supervisor: Prof.Dr. M. İlhan ÇA?mGAN December, 1997, 123 Pages The study was conducted to determine the radiosensitivity of exotic and locally adapted kabuli chicpeas to gamma rays in the Mi generation, and to identify the mutation spectrum and mutation frequency based on the segregated M2 families grown in 1995-96 in a semi-dry environment, Ürküüü town (about 37° 04' N, 30° 12' E, 1014 m above sea level), near Bucak city in Burdur province. Approximately 2000 seeds of ispanyol Population, cultivar Aydın 92, ILC 482 line, Ürkütiu native landrace and FLIP 83-47C line, were irradiated with 100, 200, 300, 400 and 500 Gy of gamma rays from a 60Co source. In the Mi generation, seedling height was determined under controlled condition and number of survived plant, percent of germination and days to maturity were recorded under field conditions to determine the mutagenic effects on genotypes in 1995. Generally, optimum dose of treated mutagen was changed from genotype to genotype. All survival plants in the Mi generation were harvested for each dose and genotype individually, the M2 generation was grown as the Mrplant-progenies sown in the Spring of 1996. In the M2 generation, different types deviating from untreated controls and from the normal looking plants within a given family for any traits were selected as potential mutants. Mutation spectrum of mutants selected in the M2 generation were clustered according to leaf and leaflet, pod and seed, plant habit, chlorophyll deficiency and flowering characters and other traits. Mutation frequency was calculated on the M2 family basis. Yield and yield-related traits such as grain yield and biological yield per plant, harvest index, plant height, number of pod, seed and branch per plant, 100-seeds weight and number of seeds per pod were recorded for comparison purposes. The correlation analysis indicated that grain yield was generally significantly correlated with biological yield, harvest index, plant height, number of pod, seed and branch per plant, 100-seeds weight. KEY WORDS: Cicer arietinum L., kabuli chickpea, induced mutation, mutation spectrum, mutation frequency, yield and yield criteria, correlation COMMITEE: ProfDr. M. İlhan ÇA?IRGAN ProfDr. Metin B. YILDIRIM Assoc.Prof.Dr. Kenan TURGUT