The visfatin localization in normal genital cycle and after the blockage of gonadotrophine releasing hormone

Except the energy storage function of the lipid tissue, it has a function like secreting the adipokines that has autocrine, paracrine and endocrine effects. The role of adipokines in obesity, insulin resistance, diabetes and cardiovascular diseases are known. It is thought that the adipokines has a potential role between ovary function and fertilization. in the present study to investigate the localization of a novel adipokine, in the genital cycle phases of the ovary. Additionally, after the blockage of folliculogenesis by GnRH antagonist, Cetrorelix, the relationship between hypothalamus-hypophysis-ovary axis and visfatin is also aimed.In the present study, 40 adult female Wistar rats were used. 5 groups that consisted of 8 rats were generated. The Prooestrus, oestrus, metoestrus and dioestrus groups were determined after vaginal smear method. 8 rats in the prooestrus stage included in the cetrorelix group. The GnRH antagonist Cetrotide was given 100 ?g/?l per rat intraperitonealy. The ovary, liver and the muscle tissues were dissected and evaluated by immunohistochemical methods.The semiquantitative evaluation of the Visfatin protein by light microscobic immunohistochemical methods indicates that the most strong staning was identified in the ovary follicles of prooestrus group. The immunostainings were around the cytoplasm of oocytes, the granulosa cells, theca cells, surface epithelium and the luteal cells. According to the H Score analyses the immunoreaction density decreases as Prooestrus, oestrus, metoestrus, dioestrus. Additionally, Visfatin immunoreaction was shown in smooth muscle fibers of ovarian artery. An intense Visfatin immunoreaction was identified in both skeleton and liver tissues.As a result, visfatin expression changes according to the ovarial cycle in rat. Visfatin expression decreases in folliculogenesis blocked by GnRH antagonist. In the skeletal muscle, smooth muscle and liver tissues the positive Visfatin immunreaction identified. We believe that Visfatin has a potential role in ovarial functions and decreases due to the hormonal decrease after the blockage of hypothalamus- hypophysis- ovary axis.