The development and application of novel hydrogen peroxide sensitive nanosensors for the determination of neurodegenerative diseases with high selectivity and sensitivity

In this work, a new type of nanosensor has been developed, which is functionalized with Tert-Nonyl Mercaptan (TNM) using a reduced-graphene oxide (rGO) and Palladium (Pd) nanoparticles (NP's). Pd-TNM@rGO nanosensor was used in the electrochemical determination of hydrogen peroxide (H2O2) at a potential range of -0.6 V to +0.8 V. The Pd-TNM@rGO sensor showed a very high activity, sensitivity, reusability and durability towards H2O2. Analytical techniques such as X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), electron energy loss spectroscopy (EELS) and raman spectroscopy (RS) were used to characterize the Pd-TNM@rGO nanosensor. The electrochemical properties of the newly prepared nanobiosensor were determined using methods such as cyclic voltammetry (CV), chronoamperometry (CA) and electrochemical impedance spectroscopy (EIS). The linear range of the prepared H2O2 nanobiosensor is between 6x10-3 mM and 1 mM. The Pd-TNM@rGO nanosensor has a detection limit of 5x10-3 M. Response time is shorter than 10 seconds. With this sensor, a new solution for lower sensitivity and selective perception of hydrogen peroxide has been put forward.