Nükleik asit temelli yanal akış testi (LFT) ile COVID-19 teşhisi

The new coronavirus, official name was determined by the WHO as SARS-CoV-2, is thought to have first appeared in the seafood market in Wuhan, China, in late December 2019. The virus is highly contagious and fatal, especially older age groups. Many studies continue to be developed for viral diagnosis. The most common and reliable one is the RT PCR recommended by WHO, and it is especially aimed at the N gene regions. This region has been important in molecular diagnosis as it is the most conserved and specific region. However, rapid tests have an important place in relieving laboratory and controlling antibody development. Among rapid tests, LFTs are the most common and practical. In long time, there will always be the possibility of viral contamination and the need for practical diagnostic tests will continue. For this purpose, our study aimed to identify the N gene regions specific to SARS-CoV-2 with a LFT platform. The LFT platform is designed on the basis of hybridization with sandwich model based on Gold nanoparticles (AuNP). The designed LFT platform was designed specifically for the conserved regions of the N gene. AuNP was conjugated with DNA oligonucleotides complementary to N gene. Sequences that will hybridize to this conjugation product were drawn on the nitrocellulose membrane as test lines. The sample containing the target sequence hybridizes with the sequences in the test lines based on the capillary flow principle when applied to the sample pad. A red color formation from AuNP can be seen with the naked eye. According to the research findings, the designed LFT specifically recognized the SARS-CoV-2 N gene regions and it was revealed that it could be an alternative to molecular diagnosis.