Investigation of the effects myricetin on mesenchymal stem cells exposed to oxidative stress

Mesenchymal stem cells (MSC) have increasingly been used both in preclinical and clinical therapies. The development of new strategies to increase the survival of transplanted MSCs is important for the therapeutic potential of stem cell-based therapies. Myricetin is a flavonoid with antioxidant, anti-inflammatory and antiapoptotic properties. We aimed to evaluate the effects of myricetin on the hydrogen peroxide(H2O2) oxidative stress model in MSC culture, immunocytochemically and electron microscopically. In the study; 4 different groups were formed as control, H2O2, myricetin and H2O2+myricetin. Cell culture viability levels of each group were evaluated with trypan blue, immunocytochemical with caspase-3, TIM17, and TNF-α antibodies, and ultrastructural level with electron microscopy. The difference between the control group and the H2O2 group was significant in terms of live and dead cell counts. A significant increase was observed between the control group and the H2O2 group in terms of the intensity of caspase-3 immunocytochemistry staining. TIM17 immunocytochemistry staining intensity was significantly decreased between the control group and the H2O2 group, while it increased significantly in the myricetin and H2O2+myricetin groups. In terms of TNF-α immunocytochemistry staining intensity, the difference between the control group and the H2O2 group was significant. As a result; considering the effects of myricetin against H2O2 toxicity damage applied to MSCs, an increase in viable cell count, decrease in caspase-3 and TNF-α immunocytochemistry staining, increase in TIM17 immunocytochemistry staining and decrease in degenerative and apoptotic cell morphology in electron microscopic evaluation were observed. Myricetin can be seen as a supportive alternative treatment option because it increases cell viability and proliferation thanks to its anti-apoptotic and anti-inflammatory effects on MSCs.