Determination of antioxidant capacities of olivetol and investigation of inhibition effects on some metabolic enzymes

In this study, in vitro inhibitory effect of olivetol against acetylcholinesterase (AChE), butyrylcholinesterase, α-glycosidase, α-amilase, glutathione S-transferase (GST) and glutathione reductase (GR), erythrocyte carbonic anhydrase isoenzymes I and II isoenzymes (hCA I and hCA II) was studied. Firstly, hCA I and II isoenzyms were purified by Sepharose-4B-L-Tyrosine affinity chromatography with 273.11 and 734.93 purification folds and %26.75 and %15.74 yields, respectively. Also, GST and GR enzymes were purified by Glutathione agarose and 2',5'-ADP-Sepharose-4B affinity chromatography techniques with 176.30, 185 purification folds and a yield of %40.45; %16,31, respectivelly. For purities of the enzymes, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was done and a single band observed for each enzyme. Then, IC50 and Ki values of hCA I, hCA II, GST, GR, AChE and BChE, α-glycosidase and α-amilase enzymes were calculated as 90.87 nM; 118.66 nM; 9.90 μM; 77.02 μM; 5.13 nM; 6.01 nM; 45.47 nM; 204.24 nM, respectively. At the same manner, Ki values were determined as 88.05±11.15 nM; 178.27±35.94 nM; 6.02±1.15 μM; 97±22.77 μM; 3.40±0.34 nM; 2.73±0.18 nM; 41.85±9.38, respectively. In the last part of this study, for evaluation of antioxidant and radical scavenging capacity of olivetol, ferrous ions (Fe2+) reducing capacity, cupric ions (Cu2+) reduction capacity by Cuprac method, FRAP reducing capacity, DPPH free radical scavenging, ABTS radical scavenging, DMPD radical scavenging, superoxide anion radical (O2•⎻) scavenging and, metal chelating activities were performed. Keywords: Olivetol, Antioxidant activity, Carbonic Anhydrase, Glutathione S-transferase, Glutathione Reductase, Acetylcholineesterase, Butyrylcholinesterase, α-Glycosidase, α-Amilase