The investigation of response of osteoblast cells to several mechanical stimulation

It is well known that not only biologic factors, but also mechanical factors affect bone cells. However the mechanism of cell response to mechanical effects couldn?t be completely determined. Cell cultures cultivated into collagen gel are designed to imitate natural in vivo situation. Because artificial synthesis with collagen has capability of being mechanically stimulated, 3-dimension and web structural. It is purposed to determine cell morphology, cell proliferation, cell alignment in scaffold after stimulation and alterations of biologic factors while applying uniaxial and cyclic strain resulting from stimulation to cells to be imitated in vivo situation and cultivated in collagen gel coated polylactic acid (PLLA). After several chemical processes a new biomaterial was manufactured with the help of polylactic acid for coating the collagen gel scaffold which is commercially being sold.A mechanical cell stimulator device operating uniaxial and cyclic strain was developed to stimulate cells cultivated into collagen gel scaffold. A stepper motor and rock gear assembled directly motor shaft in hardware of the device provides linear movement. The stimulation can be applied by stepper motor via assembling scaffold to pins located on edge of rods oriented perpendicular to gear. After osteoblast cells obtained from human bone marrow cultivated into scaffold, dynamic strain applied at 10.000 micro-strain and a frequency of 1,5 Hz, for 24 hours. DNA, protein and alkaline phosphates levels was measured to determine cell proliferation and activities of matrix synthesis of cells. Comparison with control groups osteoblast bone cells on scaffold after stimulation performed by evaluating alignment in the pores by using SEM.