Expression of the inhibitor molecules that control primordial follicle reserve decreases in ovaries after transplantation and cryopreservation

Fertility preservation techniques are crucial options for compete with infertility and hormonal insufficiency or cancer theraphies including destructive implementation such as chemotheraphy and radiotheraphy. Embryo, oocyte and ovarian tissue cryopreservation that offered by the use of assisted reproductive techniques are simplicity for preserving ovarian reserve of female patients for improving their fertility. However, even if embryo cryopreservation method has evidence-based methodology but not feasible for unmarried for prepubertal female patients. Oocyte pick-up process means waste of time for female cancer patients whose immediately need to start scheduling their theraphy. Ovarian tissue cryopreservation is unique option in some cases of tumor tissue induction risk via hormonal stimulation (e.g. estrogen receptor positive breast cancer cases). It is already known that currently methods for cancer theraphies are increased survival rate of human race. But, it's not easy to handle complications on reproductive systems. Ovarian cryopreservation is unprecedented fertility method for the group of chosen patients. Although increasing cancer incidence, a decreasing the proportion of patients who died because of cancer. Most irreversable complication from these cell destructive theraphies are damages through reproductive cells and organs. Fertility preservation theraphies would be offered to patients meanwhile and carry out with most feasible option and nearly 60 cases of gestation are announced to present day. It is restricted evidence based information about injury mechanisms due to retransplanting frozen tissue into body. Transitioning from primordial to primer follicle form, there are controlled activators and suppessors for balancing signalisation of reserving primordial follicle pool like a seesaw. In some cases of imbalancing these activators and supressors, result in activation of follicles globally then burn-out high percentage of primordial follicle pool, known as "premature ovarian insufficiency" (POI) at clinically. In respect of this, late angiogenesis and period of hipoxia may damage intrinsic mechanisms of ovary. In our study, we evaluated expressions of pTEN, Tsc1, p27 and AMH molecules for asking reasons of follicle lost after ovarian tissue cryopreservation and transplantation. Our findings show that, primordial follicles are decreasing after frozing-thawing and transplantation litterateurly correlate. We criticize expressions of pTEN, Tsc1, p27 molecules for primordial follices but growing follicles for AMH molecules. There is not much difference between K and DÇ group. Between T and DÇT group, we almost detected hardly any expressions of these molecules. With our study, we firstly point out expressions of supressor mechanisms at ovarian cryopreservation and transplantation period. Our data represents new aspects underlying process of primordial follicle activation after ovarian cryopreservation and transplantation. New functional studies about activator and inhibitor mechanisms about primordial to primer follicle transition will provide new point of view about preserving follicle pool.