A rat ovarian torsion model study:effect of nifedipin , resveratrol and detorsion treatment on serum anti-mullerian hormone levels and ovarian hystopathology

Objective: Adnexial torsion is amongst the most common gynecological emergencies which requires emergent treatment in women in reproductive age. In this study we aimed to evaluate the protective effect of detorsion, resveratrol and nifedipine treatment on AMH and ovarian histopathology. Materials and Methods: Twenty-four female, adult, healthy, nonpregnant Sprague-Dawley rats were included in this study which were separated into 4 groups: control (Group 1), torsion/detorsion (Group 2), torsion/detorsion+nifedipine (Group 3), torsion/detorsion+resveratrol (Group 4). Group 1 only received a laparotomy. 720° of bilateral ovarian torsion was performed in Group 2 and ovaries were fixed to the abdominal wall for a duration of 3 hours. Following 3 hours of ischemia, derotation was performed and rats were followed in this state for another 3 hours. At the end of 3 hours of derotation, bilateral oophorectomy was performed. In addition to the procedures performed in Group 2, Group 3 recieved 100mg/kg of nifedipine at 150 minutes and Group 4 recieved 10 mg/kg of resveratrol at 150 minutes. AMH levels, immunohistochemical expression of AMH and VEGFR-2, TAS-TOS-OSI and follicle counts were evaluated. Results: No statistically significant difference between groups in postoperative change in AMH levels were detected (p=0.058; 0.089; 0.756; 0.206, respectively). Immunohistochemical staining revealed AMH expression compared control group was similar in Group 3, significantly decreased in Group 2. Group 4 had better expression compared to Group 2 however it was still lower than in the control group. Also, in immunohistochemical staining, VEGFR-2 staining was similar in Group 2 and Group 3 but decreased in Group 4, compared to the control group. No statistically significant difference in TAS, TOS and OSI values were detected between groups (p>0.05). Primordial follicle count in Group 1 was found to be significantly higher than in Group 2 (p=0.014). Secondary follicle count in Group 3 was found to be significantly higher than in Group 2 and Group 4 (p=0.013; 0.017, respectively). Conclusion: Nifedipine and resveratrol treatments were found not to have an effect on biochemical AMH levels and antioxidant-oxidant system parameters in rats that received ovarian torsion and subsequent detorsion. Nifedipine was demonstrated to have a protective effect on immunohistochemical AMH expression and secondary follicle count. It was also observed that resveratrol decreased the expression of VEGFR-2 in tissues. Keywords: Nifedipine, Resveratrol, AMH, ovarian torsion-detorsion