The effects of ovary co-culture system to induce haploid embryo formation on eggplant (Solanum melongena L.) microspore culture

In this study, three different eggplant cultivars (Faselis F1, Amadeo F1 and Aydın Siyahı) have been used to develope an efficient protocol for haploid embryo induction. Ovary co-culture system was carried out to determine the its effects on eggplant microspore culture to induce embryo formation. The experiments were also performed to determine the optimal stage of bud development and identify the suitable microspore isolation method.To serve the purpose, first of all the most suitable microspore development stage was determined. Anthers were isolated in 0.3M mannitol solution and exposed to pretreatments for 8 days in darkness at 350C. After pretreatments, microspores were cultured in NLN medium supplemented with 5 mg/l 2,4D + 5 mg/l KIN ve 5 mg/l NAA+ 5 mg/l BAP. While no improvement was obtained in cv. Aydın Siyahı microspores, symmetrically divisions were obtained in cv. Faselis and Amadeo microspores. When wheat ovaries were added to culture medium for ovary co-culture system, isolated microspores of cv.?s Faselis and Amadeo were induced to divide symmetrically and formed multinucleated structures. On the other hand, in cv. Aydın Siyahı were induced to divide only symmetrically. No regeneration was obtained from any cultivar used under the experimental conditions.