Development of quartz tuning fork based Troponin-T biosensor by plasma polymerization method

Acute myocardial infarction (AMI) may be the reason of irreversible myocardial cell damage or ischemic necrosis in case of a sudden loss in the blood supply to myocardium. This medical case can cause the death or disability of the patient. In case of ischemia, heart muscles release some proteins including Troponin-T. The concentration of this protein levels to twice of its initial concentration in blood within the first hour after heart attack. In this study, development of quartz tuning fork (QTF) based troponin-T biosensor by plasma polymerization method has been introduced. Surface modification of quartz tuning fork made by plasma polymerization method at diffrent discharge powers and exposure times with n-heptylamine, pentylamine and 1,2-diaminocyclohexane. Contact angle of the thin films deposited on the surface has been investigated for 30 days interval. In the resistivity measurements it has been seen that the initial resistivity has been increased from 0.01 MΩ to 4.28 MΩ. Thin film properties have been characterized by scanning electron microscopy (SEM), atomic force microscopy (AFM), X-Ray photoelectron spectroscopy (XPS) and fourier transform infrared spectroscopy (FTIR). These films were to exposed Glutheraldehyde (GA) in different mediums and exposure times in order to activate the surface. Frequency shifts of these QTFs were to be measured before and after glutheraldehyde activation. These measurements both gives important data on deposited thin fils and is very important for the development of the immunosensor. After the activation of the surfaces, QTFs ere to dipped into different concentrations of bovine serum albumin and the frequency shifts were investigated. These findings will be useful for the development of mass sensitive biosensor platforms.