Quantitative analysis method development for benzalkonium chloride

Nowadays, the usage of clean-hygiene products as fast moving consumer goods which present a rapid solution is widely increasing. The most popular products of all these are wet wipes for hand cleaning. Wet wipes are produced for variety purpose such as baby cleaning, surface cleaning. Also, there are antibacterial types of wet wipes for hand cleaning. Benzalkonium chloride, benzethonium chloride, cetrimide, chlorhexidine, hexachlorophene, alcohol, hydrogen peroxide, hexamine hippurate, triclosan, cetylpyridinium chloride, dequalinium chloride are used as antibacterial agents in wet wipes. Benzalkonium chloride is quaternary ammonium surfactant, which widely used in health care and cosmetic preparations and it is also used as topical antiseptic and medical equipment disinfectants. Benzalkonium chloride which is a mixture of alkylbenzyldimethylammonium chlorides homologues, have an important biocide character. According to the usage field of Benzalkonium chloride, its concentrations are variable and it is an irritant substance when it is used in the high concentration. Particularly useful for protecting the baby's skin condition can cause discomfort, such as dermatitis. Due to these adverse effects, amount of Benzalkonium chloride has to be examined in wet wipes. In this study, a quantitative analysis method of Benzalkonium chloride was developed by using capillary electrophoresis that would become a powerful separation technique. As capillary electrophoresis was applied on different solutions of Benzalkonium chloride successfully, the homologues of Benzalkonium chloride were identified and confirmed in this study. Quantitative analysis was carried out by the external standard method. The electrophoretic separation was performed by using 75 mM pH 6.0 phosphate buffer solution with 30 percent Acetonitrile as electrolyte. The separation voltage was 10 kV, and the temperature was held at 18 centigrade degree. Samples which include 5 mg/ml Benzalkonium chloride solutions were introduced by hydrodynamic way by using 50.0 mbar pressure during 3 s. The method developed was validated according to ICH guideline and performed on wet wipes that contains 0.1 percent the concentration of Benzalkonium chloride, without any extraction productively and the calibration curves showed good linearities.