Using of the TC-99M MIBI for differential diagnosis of biliary atresia and hepatitis in rats

A total of 20 males Wistar albino rats who had weights ranging from 200-350 g were included in this study. Rats were randomly divided into 4 groups. Control group, sham operated group, biliary atresia group and chemical hepatitis group (with carbon tetrachloride) were created, respectively. Twenty images from 3 seconds (blood flow phases) and subsequently 20 images from 30 seconds (consantration and early excreation phases) dual dynamic imaging of the abdomen in anterior and posterior projection was performed after an intravenous bolus injection through the internal jugular vein of 37 MBq 99m Tc-MIBI using a gamma camera. Dual late static images were obtained at the same position at 15, 30, 60, 90 and 120 min after injection. All images were visually evaluated by two dubble blinded nuclear medicine physicians. Square region of interest was drawn over liver, heart, left kidney and bladder and mean counts were obtained on 4th and 10th images on second dynamic imaging.