The effects of different dose of exercise on some acute phase proteins and lipid profiles in rats

Acute phase response is a non-specifif response which occurs in many tissue damages and it is a quick non-specific process that protects the tissue from inflammotory events. During this reaction, o group of proteins named as acute phase proteins (APP), depending on the increase of synthesis or degredation, plasma concentrations changes at an important rate. 40 male rats are used in this study. 5 groups(n=8) are formed. Working groups are: Control 1; rats which are held in test room and no exercise programme is applied, Control 2; rats which are held in treadmill for 1 hr. First experiment group: rats which exercises 10m/min, second experiment group: Rats which exercises 2m/min and Third experiment group: Rats in the experiment group are exercised for 1 hour every day during a week. At the end of the seventh day, blood samples were collected under ether anesthesia by intracardiac way and serum samples were stored at -200C before all measurements. CRP, SAA, haptoglobin, triglyceride, cholesterol, HDL and LDL levels were measured at the same time. CRP, SAA and haptoglobin levels were analysed in ELISA reader, serum cholesterol, triglycerides, LDL and HDL levels were measured by using biochemistry otoanalizor with commercial test kits. Results were evaluated statistically. According to the data that obtained this study, exercise caused a significant increase in the levels of haptoglobin and CRP. SAA levels were also increased in rats with exercise but this increase were not significant statistically. Furthermore, acute exercise caused improvement on cholesterol, LDL and HDL but it were not cause expected changes in triglyceride levels. As a result of this study, it is determined that acute exercise caused improvement on lipid profile in rats but it also started exercise induced acute phase response. Another result of this study that acute exercise increased the concentration of CRP and haptoglobin proportionally with the exercise intensity. Keywords: Acute phase proteins, exercise, cholesterol, triglycerides, HDL, LDL rat.