The probable protective effect of lycopene on hypoksia induced oxidative stress in rats

Introduction and Aim: Today nephron sparing surgery with early diagnosis provides effective curative therapy for patients with localized renal cell carcinoma. With technological developments laparoscopic surgergy usually used on this tumors. On surgery (OPN-LPN) when renal arter temporary clemping renal ischemia-reperfusion injury occurs (temporary dialysis for patients dependent on üre and cr increased ). On this condition nephrotoxicity improved especially have a soliter kidney or determinate kidney parancime patients. The present study was designed to invastigate the possiple protective effects of lycopene against hypoksia induced renal demage. Lycopene is the one of the potent carotenoid antioxidant agent to take on foods which couldn?t synthesis on human body.Material and Method: Twelve rats are included to study from Adnan Menderes University Veterinary Faculty Laboratories, serum üre, cr, Na and K levels are detected for all rats. Famale wistar rats were diveded into two groups of six rats in each one; first group served as control, the other group were treated two days of orally lycopene (4mg/kg per day) before surgery. All wistar rats were subjected to right nephrectomy and after abdominal aorta clamping for 45 minutes for ischemia reperfusion injury. After 24 hours blood samples for taken again analysis of serum üre, cr, Na and K levels. And done left nephrectomy for biochemical and histopatholigical evaluation on Adnan Menderes University Medical Faculty Biochemistry and Pathology Laboratories.Findings: Mean of control group pre ischemia üre levels was 57,3±16,2 (34-84) mg/dL, post ischemia üre levels was 148,8±72,8 (46-229) mg/dL (p=0,046). Mean of control group pre ischemia cr levels was 0,45±0,083 (0,4-0,6) mg/dL, post ischemia cr levels was 1,17±0,97 (0,5-3) mg/dL (p=0,027). Mean of control group pre ischemia Na levels was 141,5±3,37 (136,7-145) mmol/L, post ischemia Na levels was 133,6-7,26 (122-143) mmol/L (p=0,028). Mean of lycopene group pre ischemia üre levels was 61,2±16,9 (37-86) mg/dL, post ischemia üre levels was 159±78,8 (28-241) mg/dL (p=0,046). Mean of lycopene group pre ischemia cr levels was 0,45±0,055 (0,4-0,5) mg/dL, post ischemia cr levels was 1,37±0,87 (0,4-2,8) mg/dL (p=0,046). There was no significant between pre ischemia Na levels and post ischemia Na levels on lycopene group. Mean of control group pathological score levels was 2,17±0,41 (2-3), mean of lycopene group pathological score levels was 1,55±0,55 (1-2) (p<0,05). Mean of control group tissue MDA levels was higher than lycopene group (p>0,05). Mean of lycopene group tissue GSH-Px levels was higher than control group (p>0,05).In conclusion: Ischemia reperfusion induced oxidative stress and nephrotoxicity caused significant increases in pathological score. And elavated tissue MDA levels, serum üre, cr, Na levels. Post ischemia serum Na levels was protected on lycopene group. For all results a natural antioxidant lycopene might have protective effects against hypoksia induced nephrotoxicity and utilizable on OPN and LPN.Key words: Lycopene, renal ischemia, ischemia reperfusion injury, open/laparoscopic parsiyel nephrectomy.