Reducing antibiotic resistance in biofilms via acoustic sound waves

Antibiotics are widely used to treat infections that biofilms cause. Although many bacterium species can be deteriorated due to antibiotics, most of the species can improve their defense system using excessively antibiotics as raising drug resistance. Therefore, treatment of infections should be improved via different medications as using various antibiotics. In this research, reducing antibiotic resistance by ultrasound waves has been studied. We utilized from two antibiotics which are Vabomere and Ofloxacin to destroy the biofilms. Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus hirae, Escherichia coli biofilms have been chosen for our research whose cause common chronic diseases in humans. Ultrasound waves have been applied to the biofilms without drugs to observe effects of soundwaves towards the bacteria. There were 3 main experiment group to observe changes in drug resistance and effects of acoustic sound waves. Duration of each experiment in the research was 5 minutes. In the first group, the experiments regarding ultrasound waves, whose have been applied without drugs at frequency 50 kHz, 100 kHz, 150 kHz and 200 kHz, have been performed. In the second group, both antibiotics have been injected to the biofilms to observe drug resistance and effects of antibiotics on biofilms. The last experiment group is that both antibiotics and acoustic sound waves have been applied together to the biofilms. When 5 mg dose of Ofloxacin with ultrasound waves at 50 kHz frequency have been applied to the biofilms, logarithmic reduction has been sharply decreased as a result. According to our experiments, biofilms deteriorated faster using less antibiotics and ultrasound waves at lower frequency. The antibiotic resistance has been negatively affected by acoustic sound waves at low frequency with respect to our research.