Investigation of the relationship of recombination with stress, season, and chromosomal inversions in drosophila melanogaster

In case of heterozygous chromosomal inversions, since the recombinant gametes cannot survive only the ancestral gametes can survive. In the presence of inversions inherited as a haplotype, they form adaptive clusters by reducing the recombination frequency for that region. Since it is not possible to rearrange the genome by recombination in the region where the inversion takes place, while inversions may cause a significant decrease in the recombination frequency in this region, they effect the recombination frequency elsewhere in the genome. In addition to inversions, environmental changes, especially stress factors such as heat, cold, starvation and desiccation, are known to affect the recombination frequency. Although there is strong evidence that the variations seen in recombination are adaptive, recombination has a very important place in adaptation to changing environmental conditions. Within the scope of this study, the effect of In(3R)Payne, In(2L)t, In(3R)K, and In(3L)P inversions on the recombination frequency and whether this effect had a pattern depending on the season were examined by using inbred lines collected from Yeşilöz district of Ankara at different times. Additionally, the effect of desiccation stress on recombination frequency was investigated. According to the results obtained, In(3R)Payne inversion affects the recombination frequency in both chromosome 3, which is involved in, and chromosome 2 in a statistically significant way; It has been determined that In(2L)t inversion also affects, in a statistically significant way, the recombination frequency in chromosome 2 which is involved in. Although the total recombinations occurring in chromosomes do not show seasonal variation, it has been found that certain points on the chromosome vary in recombination frequency depending on the season. In this context, the most significant seasonality occurred in the regions with the largest interval, located at the ends of the 2nd and 3rd chromosomes.