Investigation of biochemical response in gill tissue of crayfish (Astacus leptodactylus) exposed to Remazol Brilland Blue R textile dye

In this study, it was aimed to investigate biochemical response of freshwater crayfish (Astacus leptodactylus Eschscholtz, 1823) when they were exposed to Remazol Brilland Blue R being textile stain at various concentrations. The study groups were constituted as the control group (0 mg/l-1) and exposed groups to the sublethal dose of Remazol Brillant Blue R 3 (0.5 mg / l -1, 1 mg / l -1 and 2 mg / l -1). Crayfish in each aquarium were removed from the aquarium after 24 hours and 48 hours. Reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) levels in gills of crayfish were assessed for determination of biochemical response. SOD activity was investigated and there was no statistically significant difference between 24 hour and 48 hour samples (p> 0.05). It was determined that there was no statistically significant difference among the doses administered in the 24 hour sample (p> 0.05) while there was the statistically significant difference among the dose groups in the 48 hour sample (p <0.05). CAT activity showed statistically significant difference (p <0.05) both between 24 and 48 hour samples, except for 1 mg / l-1. All doses gave a significant difference between themselves when 24 and 48 hour experimental groups were compared within groups (p <0.05). GSH and MDA results were not statistically significant (p> 0.05).