The investigation of the action mechanism of ammonium pyrrolidine dithiocarbamate on rat aorta smooth muscle contraction-relaxation responses

Ammonium Pyrrolidine Dityocarbamate (APDTC) is an inhibitor of NF-κB. Besides of these functions, its antitumoral, antioxidant, anticarcinogenic and antiviral properties and also apoptosis inhibiting effect in smooth muscle cells were determined. Our experiments aimed to investigate the mechanism of action of ammonium pyrrolidine dithiocarbamate on rat aortic smooth muscle. Some adrenergic and cholinergic receptors, L-type calcium channels and potassium channels were blocked in 7 different groups, and therefore the action mechanism of APDTC whether is used or not on which channels and receptors and the extent to which they are effective were aimed to be determined. The contraction relaxation responses ffter the administration of APDTC, atropine, phentolamine, propranolol, nifedipine, tetraethylammonium (TEA) and mix (atropine+phentolamine+propranolol) on living state controlled with KCl and blocked NO synthesis with L-NAME in the pre-contraction-induced aortic preparations with phenylephrine were investigated. The obtained data were evaluated by Kruskal Wallis and Mann-Whitney U tests. APDTC created the relaxation response in the aortic smooth muscle. Cholinergic receptor blocker Atropine, α-adrenergic receptor blocker Fentolamine, β-adrenergic receptor blocker Propranolol, L-type calcium channel blocker nifedipine and potassium channel blocker TEA did not alter the relaxation response of APDTC. In the mix group consisting of atropine+phentolamine+propranolol, APDTC created a significant contraction response. It has been determined that APDTC can be effective on these systems via different mechanisms.