Evaluation of the effect of resveratrol on the healing of extraction sockets in cyclosporin A administered rats

Aim: The aim of this study is to examine histopathologicaly and immunohistochemically the effects of resveratrol (RSVL) on the healing of extraction sockets after molar tooth extractions in cyclosporine A (CsA) administered rats. Material and Method: 72 Spargue Dawley female rats were used in our study. The animals were divided into four main groups respectively: Control, CsA, RSVL, CsA+RSVL. CsA and CsA+RSVL groups received 10 mg/kg/day CsA via subcutaneous injection. Eight days following the injection left mandibular first and second molar teeth were extracted in each group. Following extraction, each of all main group were divided in to 2 subgroups. RSVL in a dosage of 10 µmol/kg were given intraperitoneally for 14 and 28 days. Animals were sacrificed 14th and 28th days postoperatively and left hemimandibles were removed and prepared for histopathological examination. Histopathological findings were statistically analyzed. Results: According to the results of our study, there was diferences in new bone formation between groups on 14th and 28th days, which was statistically significant. In RSVL administered group new bone formation was significantly more than others groups. On the other hand, new bone formation was significantly less in CsA group than others groups. Additionally, in group in which CsA and RSVL were applied together, new bone formation was similar to the CsA group and less than all other groups. Immunohistochemical analysis showed higher levels of osteocalcin and ostoepontin in the RSVL group than in the others groups. In CsA+RSVL group osteoocalcin and osteopontin levels was also significiantly higher than CsA group on 28th days. Conclusion: While CsA administration severely impaired the healing of extraction sockets in rats, RSVL administration stimulated the healing of extraction sockets. When CsA and RSVL were combined together there was increasing new bone regeneration but this was not statistically significant.