An investigation of isolation of Scenedesmus spp. from freshwater of Sinop and its culture in laboratory conditions

In order to investigate isolation and production under laboratory conditions of the Scenedesmus spp., the water samples were collected from freshwater of Sinop in spring and summer, showed a seasonal increase in number of species and they purified micropipette isolation method by using BG-11 medium after enrichment and cultures were obtained. The growth of isolated Scenedesmus spp. were followed by using BG-11 (Blue-Green Media), Bristol-Proteose medium (B-P), MWC (Modified Woods Hole Medium) and Scenedesmus medium with urea (U-S) in laboratory conditions. Cultures were incubated at 24 ± 2 °C, 65% moisture, 1800 lumen light intensity, 16 h light / 8 h dark cycling illumination. Biomass was monitored by measuring the dry weight determination, wet weight determination and chlorophyll-a content. The development of the culture by spectrophotometrically were monitored at 500, 680 and 750 nm. Biomass determined by wet weight, dry weight and chlorophyll-a values were compared four different algal media. It was observed that the spectrophotometric growth was supported with biomass results. It was determined that the development in different wavelength of isolated were found similar. Accordingly, Bristol-Proteose medium was the best growth medium, then the urea Scenedesmus medium. In the future studies on Scenedesmus spp., using of Bristol-Proteose medium that obtained maximum biomass in high cell density, best growth and high yields per unit of time is important especially for relevant other studies. In addition, Scenedesmus medium with urea could provide maximum biomass by less cost and obtain targeted products in shorter time.