Investigation of vascular endothelial growth factor and endostatin levels in some rat tissue due to cold stress and diet

Obesity, which is a disease of our age, is a condition that occurs when there is an excess of adipose tissue in the body. Obesity is not simply a concern about weight gain. Heart disease is a medical condition that increases the risk of other diseases and health problems, such as diabetes, high blood pressure, and certain cancers. In this thesis, the effects of cold stress, high-fat diet and propolis on vascular endothelial growth factor (VEGF) and endostatin levels were investigated. In the study, 36 3-month old Wistar female rats produced in İnönü University Experimental Animal Production and Research Center were used. In the study, six rats in each group were divided into six groups. Propolis administration, 2 mL of propolis dissolved in water was given to rats by gavage every day for two weeks. When the rat weights increased by 20%, euthanasia was performed and heart, liver, lung, brown and white adipose tissues were removed. After the tissues were homogenized, endostatin and vascular endothelial growth factor (VEGF) levels were measured by enzyme-linked immunosorbent assay (ELISA). In the study, while VEGF level increased in brown adipose tissue, endostatin levels decreased. Endostatin levels were increased in heart and white adipose tissue, while decreased in lung and liver tissue. VEGF levels were found to increase in other tissues except heart tissue.