Investigation of the effects of subakut metaflumisone administration on lipid peroxidation, antioxidant activity and tissues in male rats

In the thesis study, it was aimed to investigate the effects of Metaflumizon (MTF), a sodium channel blocking insecticide, on lipid peroxidation, antioxidant activity levels, liver, kidney and brain tissues in male rats. The application was started with the approval numbered 49533702/189 from the Afyon Kocatepe University Ethics Committee. In the study, a total of 32 Sprague Dawley male rats, 300-350 g, 10-12 weeks old, were randomly divided into 4 groups with 8 animals in each cage. Physiological water was administered to the control group, 50 mg/kg MTF to the second group, 100 mg/kg to the third group, and 200 mg/kg MTF to the last group by gastric gavage for 28 days orally. When the data obtained at the end of the study were evaluated, compared to the control group, the application of MTF in increasing doses increased the levels of malondialdehyde (MDA), superoxide dismutase (SOD) and catalase (CAT) significantly (p˂0.05) in erythrocyte samples, liver, kidney and brain tissues. It was determined that glutathione (GSH) levels decreased. In addition, it was reported that plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), urea, creatinine, total protein and glucose levels increased significantly (p˂0.05) with increasing doses of MTF. In addition, it was determined that histopathological changes related to damage to liver, kidney, testis and brain tissues and apoptotic findings indicating cell death occurred in MTF50, MTF100 and MTF200 groups. According to the findings obtained as a result of the thesis study, it was determined that MTF induced oxidative stress in rats and caused histopathological damage on liver, kidney and brain tissues.