Spectrophotometric determination of the aluminum with Schiff base derived from 2-aminophenol and hydroxy benzaldehyde

In this thesis; a sensitive, highly selective and visible region-absorbing complex has been formed by using synthesized and characterized (E)-2-((2-hydroxybenzylidene) amino)-4-methylphenol (8S4) ligand and a new UV-Vis (Visible region) spectrophotometric method was developed for the spectrophotometric determination of trace amounts of aluminum ion. Optimum pH of the complex is 5 and maximum wavelength is 407.00 nm. The complex stoichiometry was determined using the Job method and the metal ligand ratio was found to be 1:2. The method obeys Lambert Beer's law in the concentration range of 0.137-1.079 μg mL-1. The calibration graph equation and r2 (correlation coefficient) values are A =0.0492C (μg 10mL-1) + 0.069 and 0.9971. The molar absorption coefficient and Sandell's sensitivity are 1.78x104 Lmol-1cm-1 and 1.516x10-6 μg cm-1, respectively. The limit of detection (LOD) and the limit of quantitation (LOQ) were calculated as 0.076 μg mL-1 and 0.023 μg mL-1, respectively. The selectivity of the method was examined and it was observed that many anions and cations did not interfere. The developed method was successfully applied to the determination of aluminum in two different artificial samples, drinking and tap water samples. In addition, the solvatochromic behavior of the 8S4 ligand was investigated by the UV-Visible spectrophotometer. Keywords: Schiff base, UV-Visible region spectroscopy, Solvathochromic behavior, Spectrophotometric determination, Aluminum determination.