Determination of the genetic diversitybetween some rice varietiesdeveloped in Turkey using ISSR technique

In this study, the genetic diversity levels of 28 different Rice varieties developed in Turkey were determined by using ISSR markers. We used 20 different ISSR primers and obtained 268 bands of which 217 were polymorphic. Band size varied between 250 bp and 2500 bp. Band numbers for each primer produced varied between 5-22 with an average number of 13.4. Bands were recorded as present (1) or absent (0) and similarity matrix was created by using NTSYS Pc 2.0 software and a dendrogram was constructed based on UPGMA technique. All primers used showed polymorphism. Polymorphism percentages varied between 40.00% and 94.12% with an average of 80.97%. Polymorphism Information Content (PIC) values varied between 0.274 and 0.374 with an average value of 0.347. As a result, the most similar varities were found as Efe and Hamzadere (0.856) and the least similar varieties were found as Demir and Bafra Yıldızı (0.395). The results obtained from this study is thought to contribute the ongoing rice improvement programs. Key Words: Oryza sativa , Rice, ISSR-PCR, Genetic Diversity